Effect of Laser Diode 820Nm on the Levels of Lactate Dehydrogenase, Creatin Phosphokinase, and Lactic Acid in Muscles of Students

Lactic acid is the end product of glycogenolysis. Glycogen is converted to pyruvic acid in the deficiency of oxygen. The creatin phosphokinase enzyme activity is important to change AMP to ATP in glycogenolysis. However, in the deficiency of CPK, the pyruvic acid is changed to lactic acid with the lactate dehydrogenase (LDH) enhancement. Low level laser treatment (LLLT) is one of the most important research aspects targeting the cellular level. The output laser should be low and do not make thermal changes inside tissues. The aim of this study was to see the effect of laser diode 820nm on the levels of lactic acid and body enzymes, lactate dehydrogenase (LDH) and creatin phosphokinase (CPK), in the after physical activity of facial muscles which are considered important for various activities.

The sample was taken from the 1^st-year dentistry students, College of Dentistry/Kufa University in 2014–2015 in collaboration with Al-Saddar Teaching Hospital, Kufa City, Iraq. This study was based on retrospective studies that are related to our research. The protocol of this research was based on clinical studies on three groups of students. Each group contained 30 patients. All the samples were based on same biological qualification of age, weight, and length. The first two groups were submitted into pre and post evaluation of the lactic acid, LDH, and CPK except the control group. From all the groups, each subject was asked to chew a gum of frankincense continuously for a half hour. The weight of the gum was 1.5gm.

The result of this study was positive toward the laser group. After mouth muscle activity, lactic acid and enzymes accumulated in the muscle cells. The laser effect reduces muscle fatigue and pain due to reorientation the chemical levels to normal values.