1Chemistry and Biochemistry Department, College of Medicine, Al-Nahrain University, Baghdda, Iraq
2Department of Pediatrics, College of Medicine, Al-Nahrain University, Baghdda, Iraq
Asthma is a perpetual provocative sickness comprising a mix of natural and hereditary factors. The catalyst prolidase assumes an imperative part in the breakdown of collagen, the breakdown of intracellular protein particularly in the last stage when peptides and dipeptides contain an abnormal state of proline. The aim of current study was to estimate prolidase enzyme levels in serum, plasma and hemolysate of asthmatic children and their healthy control.
A case-control study included two groups; the first group consisted of (80) asthmatic children aged 1–13 years. The second group contained 30 children as control. Enzyme activity was measured using spectrophotometry.
The serum levels of prolidase enzyme showed significant decrease in asthmatic children group in comparison with control group (P<0.001). Also, serum levels of prolidase enzyme were significantly decreased with increasing severity of asthma in patients subgroups (P<0.001).
Spectrophotometer method is a simple and good method for determination of prolidase enzyme activity in patients with asthma.
Prolidase, enzyme activity, spectrophotometry, asthmatic children, AUC, sensitivity, specificity
