The Cytotoxic Effects of Lipopolysaccharide Extracted from a Local Isolate of Salmonella enteritidis on Breast and Ovarian Cancer Cell Lines

The current in vitro study was performed to evaluate the anti-tumor cytotoxic effects of lipopolysaccharide (LPS) extracted from a local isolate of Salmonella enteritidis on breast and ovarian cancer cell lines, MCF7, SKOV-3 respectively. Here, a local isolate of S. Enteritidis was obtained from Al-Diwaniyah Teaching Hospital, Al-Diwaniyah City, Iraq. The isolate was exposed to a series of morphological examinations and API 20E kit-based test to confirm the identity of the bacterium. Then, the bacterium was used in a polymerase chain reaction (PCR), and partial sequencing is targeting the RNA polymerase subunit beta (rpoB) gene at pieces of 1090bp and 897bp respectively to confirm the bacterium identity. After that, the bacterium LPS extracted to study its cytotoxic effects against MCF-7 and SKOV-3 cancer cell lines, in comparison with a commercial LPS using different concentrations (100μg/ml, 200μg/ml, 300μg/ml, 400μg/ml, and 500μg/ml). The results of the cytotoxicity effects showed that both LPSs had significant (p˂0.05) cytotoxicity against both cell lines. This study reveals that LPS isolated from Salmonella enteritidis might provide a promising substance for controlling breast and ovarian cancers.