Role of Toll Like Receptor-9 Gene Polymorphism among Patients with Exacerbation Chronic Obstructive Pulmonary Disease in Holy Karbala, Iraq

There are few studies investigate the effect of TLR-9 (T1237C) gene polymorphism in the development of Exacerbation Chronic Obstructive Pulmonary Disease (ECOPD), therefore, this study mainly aims to estimate whether (T1237C) polymorphism in TLR-9 gene is associated with ECOPD in Iraqi patients. Case Control Study was done on patients were diagnosed clinically and confirmed with spirometer test. Attended AL-Hindia hospital and Imam AL-Hussein medical city hospital in Karbala/Iraq. The sampling collection period extended from October 2017 to April 2018, the study included 40 patients with ECOPD and 40 as a healthy control. The permission had been taken from all subjects, venous blood sample with EDTA tube collected, PCR performed for TLR-9 gene detection followed with RFLP technique for revelation of SNP (rs5743836)(T1237), the distribution of genotypes and allele frequency was tested for differences between controls and ECOPD patients. We observed in present study, there were three genotypes for this SNP among ECOPD patients; CC, TC and TT with frequency of 5 (12.5%), 27 (67.5%) and 8 (20%) respectively, whereas, there were only two genotypes among control group; TC and TT with frequency of 17 (42.5%) and 23 (57.5%) respectively.