Study to Diagnose the Entamoeba histolytica Parasite from Patients with Diarrhea Attended to General Education Hospital in Al-Qadisiyah Province Use PCR Conventional for Detecting

The present study designed to diagnose the Entamoeba histolytica parasite from patients with diarrhea attended to General Education Hospital in Al-Qadisiyah province, Aged between 18 to 40 years at the period from the beginning of March to the end of December 2017. The number of samples that have been collected (210) stool samples, and the number of samples infected is (72) sample; After samples were examined in direct wet smear method using normal saline, include the study to investigate and to emphasize the presence of virulence factors amoebapore in parasite using Conventional PCR Technique. The result showed that the infection rate was 34.28%. The age group of 24–29 years showed the highest rate of infection(33.33%), while patients aged 35–40 years showed the lowest rate of infection(18.05%). The infection rate in male was higher (58.3%) than in female (41.6%). It was found that the majority of cases (63.88%) were from Urban areas. After amplified using special designed for primers E. histolytica virulence factor genes that was called amoebapore for DNA. The results showed that the mentioned factors is present in all positive samples of E. histolytica.