An Evidence for Ability of Malassezia Species to Experimentally Induce Pityriasis Versicolor in Various Skin Layers by Using Periodic Schiff Stain(PAS)

The fungal agents were visualized in skin sections stained with Periodic Schiff Stain (PAS), fungal spores and hyphae were attached and colonized in the stratum corneum of most of skin sections from infected rat skin with Malassezia species spores isolated from patients, spores well organized, morphologically identified and this method very suitable for diagnosis of Malassezia species from other pathogenic yeasts, the present results showed Malassezia species yeasts that penetrated the corneum layer formed sometimes sheet or aggregation on epidermis surface with cellular debris. Moreover the spores budding noticed, the hyphae showed filamentous with pink color.