Indian Journal of Public Health Research & Development

The effect of TiO₂ nanoparticles on Pseudomonas aeruginosa was determined and the results observed that this compound had inhibitory effect on the isolate (1) of bacteria but not with isolates (2, 3). Moreover the effect of Olea europea leaves extract on Pseudomonas aeruginosa was studied, the results of this study showed that there was inhibitory effect of olive leaves extract against the three isolates of the studies bacteria (1, 2 and 3) with different rates of inhibitory zone. When TiO₂ nanoparticles mixed with olive leaves extract the results were higher in effect and the mixture showed a synergistic action against the three isolates of Pseudomonas aeruginosa. The detection results of the active compounds revealed that the hot crude alcoholic extract of olive leaves contained flavones, tannins, alkaloids, phenols, glycosides, fatty acids, steroids and terpins. The X-Ray Diffraction Analysis (XRD) of TiO₂ was performed also for TiO₂ solution, and the results showed that the average size of each molecule of TiO₂ nanoparticles was (15.96nm).