Indian Journal of Public Health Research & Development

This study mainly aims to evaluate whether Thr399Ile polymorphism in TLR-4genes is related to DFU in a sample of Iraqi patients with type 2 diabetes mellitus (T2DM). The case-control study included 120 patients with the type 2 diabetes, 60 participants with the type 2 diabetes and foot ulcer group I, 60 participants with the type2 diabetes group II and 60 controls group III.TLR-4(Thr399Ile) genotyping was done by Polymerase chain reaction (PCR) followed by restriction analysis. There was no statistical difference in the distribution of TLR-4 (Thr399Ile) genotypes between the 3 study groups, the genotypes in group I included CT 6 (10.0%) and TT 0.(0%) was compared with wild type CC 54.(90.0%) while CT 10(16.7%) and TT 0.(0%) was compared with CC 50.(83.3%) in group II and in group III CT 7(11.7%) and TT 0.(0%) was compared with CC 53.(88.3%).The distribution of genotypes between group I and group III was (OR= 1.2, 95% CI: 0.4–3.8, p= 0.8) while when comparing group I and group II (OR=1.8, CI=0.6–5.3, p=0.3). On the other hands, the comparison between group II and group III was (OR= 0.7, 95% CI: 0.2–1.9, p = 0.4).