Effect of Adding Probiotic with Different Levels of Citric Acid in Feed Supplementation to Laying Hens on Bacteria Content of Feed, Litter and Egg Shell

Study conducted to determined effect of adding Probiotic (Iraqi Probiotic) with different levels of citric acid to Lohmann brown laying hens in order to know the effect of addition on the microbial content of the feed, litter and eggshell. The treatments were T1 without any addition (control), T2 (0.25) probiotic, T3, T4 and T5 add Probiotic each with a fixed level of 0.25% with levels 0.15, 0.30, 0.45% of citric acid sequentially. The Addition of probiotic with citric acid led to a significant decrease (p <0.05) in both (Total count bacteria) and (Coliform bacteria) in feed and litter compared with the control treatment and the treatment of adding probiotic alone. The addition of either alone or with citric acid lead to a significant decrease in the total number of aerobic bacteria on the eggs product and no effect on the number of E.coli in the eggs product.