Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 7

Inorganic Nitrogen Salts in Fibrinolytic Enzyme Production from Bacillus Megaterium BM 9.1 with Solid State Fermentation

1Pharmacy Department Faculty of Pharmacy, Airlangga University, Jl. Airlangga No 4–6, Airlangga, Gubeng, Surabaya city, Jawa Timur

Abstract

The use of fibrinolytic enzymes is necessary as a thrombolytic agent in the treatment of vascular disorders. Most fibrinolytic enzymes are obtained from fermented food bacteria.

This study was conducted to determine the effect of inorganic nitrogen salt against the production of fibrinolytic enzyme from Bacillus megaterium BM 9.1.

This experimental study was first conducted on Bacillus megaterium BM 9.1 from coastal waters which were overgrown with mangrove trees. Media preparation used consisted of 3 types, namely nutrient agar, nutrient agar with the addition of inorganic nitrogen salts (KNO3, NaNO3, (NH4) 2SO4 and NH4Cl) and agar with addition of inorganic nitrogen salts (KNO3, NaNO3, (NH4) 2SO4 and NH4Cl).

From 9 media that have been treated, 100% of them positive in producing fibrinolytic enzymes. Nutrient Agar (NA) + KNO3 is the best medium with the highest Fibrinolytic Index (IF) mean, which is 5.60 ± 0.087. Determining the optimum concentration of potassium nitrate does not mean the highest concentration are having the high influence. The optimum fibrinolytic activity of Bacillus megaterium BM 9.1 are found at 2% potassium nitrate concentration.

Fibrinolytic enzymes can be easily found from some fermented foods, especially the Bacillus (genus Bacillus). Bacillus megaterium BM 9.1 in the inorganic nitrogen salts is one of the agents that produce fibrinolytics enzymes which can be used as a therapy for fibrinolytics agents.

Keywords

Bacillus megaterium BM 9.1, Inorganic nitrogen salts, fibrinolytic activity, solid state fermentation