Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 8

Differences of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Moringa Leaf Extract (Moringa Oliefera L.) on Bacteria Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis

  • Author:
  • Arni Irawaty Djais1, Hasanuddin Tahir1, Mochammad Hatta2, Harun Achmad3,, Ainul Wahyuni4
  • Total Page Count: 5
  • Page Number: 896 to 900

1Department of Periodontology, Faculty of Dentistry, Hasanuddin University, Indonesia

2Department of Microbiology, Molecular Biology and Immunology Laboratory Faculty of Medicine, Hasanuddin University, Makassar, Indonesia

3Department of Pedodontic, Faculty of Dentistry, Hasanuddin University, Indonesia

4Dental Hospital, Hasanuddin University, Faculty of Dentistry, Indonesia

*Corresponding author: Harun Achmad, Department of Pedodontic, Faculty of Dentistry, Hasanuddin University, Indonesia

Online published on 26 September, 2019.

Abstract

Moringa leaves (Moringa oliefera L.) has many nutrients that contain bioactive components such as tannins, flavonoids, and saponins, as antimicrobials. The growth of Aggregatibacter actinomycetemcomitans and Porphyromonas Gingivalis must be inhibited so that they do not become pathogens and cause periodontitis.

The general purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) and inhibitory power of moringa leaf extract (Moringa oliefera L.) against bacteria Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

The type of research used was laboratory experimental research. The design of this study is post test only control group design using the Kirby Bauer dilution method. With treatment extract concentration of 15%, 20%, 25%, 30%, 35% 100%, and positive control (Metronidazole). The measuring instrument in this study uses a caliper with millimeters (mm).

The results of the study of bacteria Porphyromonas gingivalis obtained from MIC by 20%, MBC 25% and bacteria Aggregatibacter actinomycetemcomitans obtained by MIC by 30% and MBC 35%. Kruskall Wallis test results showed that the value of p<0.05 so it can be concluded that there are significant differences in the inhibition zone of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

The extract of leaves of Moringa (Moringa oliefera L.) were able to inhibit bacterial growth Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis.

Keywords

Moringa leaves extract (Moringa oliefera L.), inhibitory power, bacteria Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis