Faculty of Science, University of Kufa, Departement of Biology, Iraq
*Corresponding author: Attyaf Jameel Thamir Al-Tamimi, Faculty of Science, University of Kufa, Departement of Biology, Iraq, E-mail: atyaf.altameemi@uokufa.edu.iq
Online published on 26 September, 2019.
Cultivar identification is an essential step for crop improvement because it can direct plant breeders for sauitable crossing to get heterosis, this study designed to use seed total protein, RAPDs and SSR to evalute their ability in cultivar fingerprinting and identification
Seven pea genotypes with divese origin were used. As biochemical marker, total seed protein extracted and profile etablished using SDS-PAGE. Genomic DNA extracted and used to examine ten of RAPDs primer and three SSRs were used as DNA markers and ampilfied by PCR followed by agarose gel electophoresis, photographs and data analysis
Low genetic variation was observed using SDS-PAGE among studied genotypes. Primer OPA-04 successfully fingerprinted all genotypes. Primer PSMPA5 was the most efficient in all studied aspects among SSRs markers. No match observed between Phylogenetic analysis of RAPD and SSRs with genotypes origin
Molecular markers proceed biochemical markers in fingerprinting and identification pea cultivars.
Pea, SDS-PAGE, SSRs, RAPDs
