1Department of Public Health, College of Veterinary Medicine, Al-Basra University, Basra, Iraq
2Department of Pathology and Poultry Diseases, College of Veterinary Medicine, Al-Qadisiyah University, Al-Qadisiyah, Iraq
3Department of Microbiology, College of Veterinary Medicine, Al-Qadisiyah University, Al-Qadisiyah, Iraq
4Zoonotic Diseases Unit, College of Veterinary Medicine, Al-Qadisiyah University, Al-Qadisiyah, Iraq
*Corresponding Author: Jalal Yaseen Mustafa, E-mail: dr.jalalyaseen1982@yahoo.com
Online published on 26 September, 2019.
Influenza is a worldwide zoonotic disease caused by Orthomyxoviridae virus. The appearance in humans of the A (H1N1) pdm09 influenza virus, a complex reclassified virus of swine origin, highlighted the importance of worldwide influenza virus surveillance in birds. To date, wide-control surveillance studies have been reported for Asia and North America, but such deep data have not yet been described in Iraq.
One hundred forty-five (145), human samples used in the study were \collected from General Teaching Hospital and Specialized Hospital Maternity and Children Teaching Hospital and Center Pulmonology and Respiratory Specialist in Al-Diwaniyah city following the WHO (15) influenza-like illness case definitions. A total of 145 clinical samples (throat swabs and nasopharyngeal swabs [73], bronchial lavage [72]) collected in December 2015 to January 2016 (2 Months period) and evaluated for the presence of influenza A (H1N1). Chicken tracheal swabs were (113) Sample) from different broiler flocks in Al-Diwaniya. All samples tested directly by using taq man Real Time-PCR technique.
Showed that the infection incidence with influenza A(H1N1) pdm09 in humans (18%) and in broiler (65.48%), the study proved that there was a significant difference at the site of infection in the respiratory tract (URT& LRT) for humans, as well as in terms of age groups.
Influenza is a highly potential zoonotic circulating disease between peoples in Al-Diwaniyah city and clearly defined risk groups: people with respiratory infection, heart and vascular disease, immunosuppressed patients and distributed in chicken in broiler flocks that have respiratory disorder. Real Time-PCR is a potential tool in diagnosis of A(H1N1) pdm09 virus between human and chicken
A(H1N1) pdm09, human, broiler, Taman real time-PCR, respiratory diseases, & Iraq
