1Division of Pediatric Surgery, Departement of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
2Departement of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
3Post Graduate of Medical Science, Faculty of Medicine, University of Hasanuddin, Makassar, Indonesia
Online published on 26 September, 2019.
HAEC is a condition of inflammatory bowel which has clinical characteristics such as fever, abdominal distension, diarrhea, foul-smelling stools and sepsis. At Hirschsprung Disease autopsy that has HAEC, crypt abscesses, mucous ulcerations, and transmural necrosis are seen. In the pathogenesis of inflammatory bowel disease, intestinal flora may also play a key role in the pathogenesis and development of HAEC. CD18 leukocyte disorders and T cell regulation can be associated with genetic predisposition (ITGB2) for HAEC.
The aim of this study was to investigated the ITGB2 (CD18) mRNA expression in a cohort of patients with Hirschsprung-associated enterocolitis (HAEC)
Screening for ITGB2 (CD18) mRNA expression was performed on DNA extracted from colonic tissue samples of 30 HAEC patients from the Makassar, South Sulawesi, Indonesia population. Polymerase chain reaction amplification was performed, followed by heteroduplex single-strand conformation primer analysis and bidirectional semiautomated DNA sequencing analysis.
From 30 HAEC patients; 25 (83, 3%) male patients, 5 (16, 7%) female patients. 13 (43, 3%), aged ≤ 1 year, 3 (10, 0%) aged 4–5 years. The value (p-value <α) or (0.038 <0.05) can be concluded that there is a relationship between ITGB2 mRNA expression (CD18) and Genesis HAEC, it can be concluded that the lower the ITGB2 mRNA expression (CD18) then the HAEC event will be increase.
There is a relationship between ITGB2(CD18) mRNA expression and the occurrence of HAEC, the lower of ITGB2(CD18) mRNA expression, the higher histopathological severity degree of HAEC.
Hirschsprung's-associated enterocolitis (HAEC), mRNAITGB2(CD18), Hirschsprung's disease
