Department of Biology, College of Education for Pure Sciences, University of Kerbala, Karbala, Iraq
Asthma is a chronic respiratory disease that affects the lungs, impairing air from reaching the lungs, leading to shortness of breath and severe cough. This disease is caused by environmental and genetic factors include change in CD14, MnSOD genes and others. CD14 is a high-affinity receptor for various bacterial products as lipopolysaccharides (LPS) and acts as a vector of inflammation. Blood samples were collected from 80 patients with asthma after being diagnosed by the specialist at Imam Hussein Medical Teaching Hospital, holy Karbala, Iraq, and compared with 50 apparently healthy individuals as a control group. DNA was extracted from blood samples for all study samples. Molecular detection of single nucleotide polymorphisms (SNPs) was performed in genetic sites using PCR and PCR-RFLP techniques. CD14 gene levels showed significant differences between the patients and control groups in the CC model, while no significant differences were found in CT and TT genotypes. The study showed no significant differences between the genotypes (Ala-Ala, Val-Ala, Val-Val) in patients and healthy group. The study examined the effect of certain factors such as age, sex, place of residence, smoking, family history on the rates of asthma to determine their relation to the polymorphism of the studied genes.
PCR, PCR-RFLP, CD14, MnSOD, ROS, Asthma
