Department of Biology, College of Education for Pure Science, University of Wasit, Iraq
Online published on 26 September, 2019.
The regulation of immune system at multiple levels is related with IL-4. Diabetes is a global health problem and plays an important role in the high mortality rates in the world.
In current work the mutational changes related with of IL-4-590 C>T gene polymorphisms were analyzed among 64 type-2 diabetic patients (T2DM) and 26 individuals as control group. Patients and controls were from Wasit province in Iraq.
The study design was case-control study.
The allele-specific PCR (ARMS-PCR) technique was used to detect the IL-4-590 C>T gene polymorphism (rs2243250). PCR products for IL-4-590 C>T and primers were prepared for sequencing analysis in order to detect mutations and alterations in IL-4-590 C>T gene of T2DM patients.
After alignment of product amplification of IL-4 590C>T CC or CT genotypes, there are nine transition substitution mutations in patients with diabetes mellitus. Transition substitution mutations: Trannsition substituation mutations: six transition substitution mutation adenine to guanine in site 721, Guanine to adenine in site 858, and, guanine to adenine in site 858.
We conclude that different transition mutations at IL-4 gene 590C>T are associated with type-2diabetes mellitus.
Interluekin-4, diabetes melltus, polymerase chain reaction, transition, mutation
