Evaluation on Heavy Metals and Microbiological Contamination in Instant Papeda with Laor Koya (Polychaeta) as Supplementary Food for Pregnant Woman

Having a safe meal is the human rights. In fact, heavy metals and natural pathogenic microorganisms often contaminate the food people consume. Heavy metals and harmful microorganisms could ease the disease transmission with the food as the media.

This study aims to evaluate some of the heavy metals and microbiological contaminants on the formula of instant papeda enriched with laor (Polychaeta).

Laor (Polychaeta) is substituted to Skipjack Tuna with a ratio of 100%: 0% (PI); 80%: 20% (PIKL1); 70%: 30% (PIKL 2) and 60%: 40% (PIKL 3). Atomic Absorption Spectrophotometry (AAS) was used to determine metal contamination. Total Plate Count (TPC) was used to determine the contamination numbers of total plate count. Most Probable Number (MPN) method determined Coliform. Isolation and identification method were used to determine Escherchia coli, Salmonella, and Staphylococcus aureus. One Way Anova test.Confidence Interval (CI) 95% was used to discover statistical analysis for metal contamination. Microbial contamination is descriptively analyzed.

Substances level found in instant papeda with laor koya are as follows: Pb (Lead), the lowest level: 0.0106 mg/kg, the highest level: 0.011 9 mg/kg. Cu (Copper), the lowest level: 0.0037 mg/kg, the highest level: 0.0075 mg/kg. Sn (Tin), the lowest level: 0.0062 mg/kg, the highest level: 0.0087 mg/kg. Hg (Mercury), the lowest level: 0.0015 mg/kg, the highest level: 0.0017 mg/kg. Total microbial contamination is 0 colony/mL. MPN coliform states 0 colony/mL, Staphylococcus aureus is 0 colony/mL, Escherichia coli and Salmonella shows negative result.

The metal emission does not exceed the maximum limit stated in Indonesian National Standard (INS) or Standard Nasional Indonesia (SNI) and Codex and there is no microbiological contaminant found in instant papeda with laor koya.