1University of Al-Qadisiyah, Iraq
*Corresponding Author: Osamah Abd Ali Nassr, University of Al-Qadisiyah, Iraq, Email: osama.alshafeay@gmail.com
Online published on 26 September, 2019.
Colorectal cancer is the most common malignant tumor of the lower gastrointestinal tract (colon and rectum) of epithelial origin, perhaps the only cancer that starts as a benign adenomatous polyp, which can last a few years to become cancer. The RAS gene family is among the most studied and best characterized of the known cancer-related genes. Of the three human ras isoforms, KRAS is the most frequently altered gene with mutations occurring in 17%-25% of all cancers. The aim of current study was to develop a highly sensitive method for detecting somatic mutations of KRAS gene for genotyping CRC patients.
the present investigation included 20 control group and 40 patients with Colorectal Cancer with ages between (26–82 years) for the identification of KRAS gene by symmetric PCR technique (RT-PCR). The risk of mutant KRAS genotype in association with colorectal cancer was assessed in terms of Odds ratio.
Current study showed that persons harboring mutant KRAS genotypes are at two-fold risk for developing colorectal carcinoma than the general population.
On the basis of current study findings, significant over productions of KRAS mutations seemed to have potential role in colorectal cancer progression and worse prognosis when compared with healthy people
Colorectal Cancer, KRAS gene, genotyping, mutations, prognosis
