1Al-Furat AL-Awsat Technical University, Iraq
2University of Kufa, Iraq
3Collage of Medicine, Kerbala University, Iraq
*Corresponding Author: Saba Fadhil Ali Malaa, Al-Furat AL-Awsat Technical University, Iraq, Email: sabafam6@gmail.com
Online published on 26 September, 2019.
About ten percent of the world's people are infected with Entamoeba. histolytica, which representing over fifty million people annually. Sequences of 18S ribosomal RNA gene have been obtained for five new isolates of Entamoeba histolytica. which is represented new insights into the evolution of phylogenetic analyses of this parasite in Iraq. In the current study, the phylogenetic relationships between five local isolations of Entamoeba histolytica and eight Entamoeba spp global isolates were studied by using the18S rRNA gene sequence.
The analysis of multiple sequence alignment and the adjoining phylogenetic tree analysis was carried out by using ClustalW multiple sequence alignment online based analysis of 355bp 18S rRNA gene was amplified by Polymerase Chain Reaction.
Phylogenetic analysis results of these gene sequences revealed that Entamoeba histolytica local isolations were closely related to Entamoeba histolytica two Japan isolation (AB282660.1) and (AB485592.1) more than other countries. Accession No: KT253450, KT253451, KT253452, KT253453 and KT253454, these isolates are unique, and a new strain, this is the first report on the usage of molecular phylogeny to classify Entamoeba histolytica which is found in Babylon Province of Iraq.
Entamoeba histolytica, phylogenetic tree, Polymerase Chain Reaction, 18S rRNA gene sequence
