Indian Journal of Public Health Research & Development

  • Year: 2019
  • Volume: 10
  • Issue: 8

In vitro Experimental Research for Using the Silver Nanoparticles as Plasmid Curing Agent in Some Types of Multi-Antibiotic Resistant Pathogenic Bacteria

1Dept. of Med. and Mol. Biotech., College of Biotech, Al-Nahrain University, Iraq

2Dept. of Applied Sciences, Division of Biotech., University of Technology, Iraq

Abstract

Nanomaterials, between the present scenarios, bear born new avenues in medicine, diagnosis, or therapeutics. In try concerning this, the present-day discipline goals in imitation of deciding the working efficiency concerning silver nanoparticles in accordance with treatment plasmids of bacteria.

A total of sixteen multi-antibiotic resistant isolates made up of 4 (25%) Escherichia coli, 4 (25%) Pseudomonas aeruginosa, 4 (25%) Staphylococcus aureus and 4 (25%) Streptococcus pyogenes were collected from different samples of burn and wound infections from patients attended to Al-kadhemyiah Teaching Hospital/Baghdad/Iraq. These strains were diagnosed molecularly by PCR on 16S rRNA using primers named 27F and 1492R; which yielding of 1, 500bp or extra as shown with sequencing data.

Strains showed multi antibiotic resistance to Gentamycin, Ciprofloxacin, Ofloxacin, Ampicillin, Nitrofurantoin and Erythromycin respectively. Resistance plasmid DNA of multi-antibiotic resistant strains used to be cured with three different concentrations of 0.75, 1.5, and 3 ppm of silver nanoparticles were confirmed by PCR technique for plasmids curing assay where, at 0.75 ppm of silver nanoparticles the plasmid curing percentage relatively were 50%, 75%, 100% and 100% respectively while at 1.5 ppm plasmid curing percentage were 75%, 50%, 100% and 100% and at 3.0 ppm plasmid curing percentage were 100%, 100%, 100%, 100% for (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Streptococcus pyogenes respectively) and cured traces have been again subjected to in vitro antibiotic sensitivity trying out to confirm curing actions.

Keywords

Antimicrobial resistance, multi-antibiotic resistance, nanoparticles, silver