1Department of Medical Laboratory Techniques, Al-maarif University College, Al-Anbar, Iraq
2Institute of Medical Technology, AL-Mansour, Baghdad, Iraq
3Alnokba Laboratory Diagnosis, Baghdad, Iraq
4Biotechnology and Immunology Department, Al-Nosor University, Baghdad, Iraq
*Corresponding Author: Raed Obaid Saleh, Department of Medical Laboratory Techniques, Al-maarif University College, Al-Anbar, Iraq, Email: raedalmaarif@auc-edu.org
Online published on 26 September, 2019.
The oral cavity is considered as a complex microbial community. Streptococcus mutans has been the major bacteria that cause dental caries. This species of bacteria have many factors to facilitate colonized the tooth surfaces causing caries and lesions.
In this study, (150) samples include buccal swabs, which were randomly collection from 150 patient′ oral cavitites (75female and 75male) during the period from November 2017 to April 2018 from non-government dental clinical in Baghdad, Karkh and Rusafa. The age of patients ranged from 25 to 50 years. The samples were cultured on selective and enriched culture media. Then, Streptococcus mutans isolates were identified by using Polymerase Chain Reaction) PCR) technique and sequencing of gtfD gene.
The results of the sequencing showed congruence with isolation Streptococcus mutans of amplified product of gtfD gene appeared 93%compatibility. (540to807) number of nucleotide from the gene of a gene bank, and have number score (403) bits, expect (1e-108), and corresponds to the global number ID: JX073019.1.
PCR method was used to detect gtfD gene in buccal swabs which is more sensitive than API 20 strepts and culture media to identify Streptococcus mutans. The study showed that oral Srept. mutans isolates had gtfD gene encoding glucosyltranferase which responsible for Streptococcus mutans pathogenicity and causing dental cavies in different age groups.
Dental caries, Oral Streptococcus mutans, PCR technique, gtf gene
