Indian Journal of Public Health Research & Development
  • Year: 2019
  • Volume: 10
  • Issue: 8

Molecular Study of Toxoplasmosis in Spontaneous Aborted Women in Thi-Qar Province, Iraq

1Dept. of Medical Lab. Tech, College of Health & Medical Technology, Southern Technical University, Iraq

2Dept. Biology, College of Pure Science, University of Basrah, Iraq

*Corresponding Author: A.H. Awad, Dept. Biology, College of Pure Science, University of Basrah, Iraq, Email: abdul_hussein2009@yahoo.com

Online published on 26 September, 2019.

Abstract

Toxoplasma gondii infection can be diagnosed with different methods. The most important one is a Polymerase Chain Reaction (PCR), which is used for the detection of T. gondii DNA. This method is simple, sensitive, reproducible, cost-effective, and used in a variety of clinical samples from animals or humans.

In this study a blood sample of 305 abortive women were collected from Bent AL-Huda and Al-Hussian Hospitals in Thi-Qar province from October 2017 till October 2018. Sample, which gave positive results (133) with ELISA test were used in PCR technique. DNA extraction of parasite was evaluated by using specific primers for Toxoplasma gondii. (193 bp, 580 bp, 399 bp).

The PCR results of aborted women showed that the Positive IgG was found in (34) sample 25.6%, IgM+ in (14) sample 10.53%, both IgM; IgG in (6) sample 4.51%. The high infection rate was found at age group (25–29), and the lowest at age group (30–34) years and according to residency rural (29%), and urban (47.6%). The positive PCR in first trimester was (39.62%), in second trimester was (47.36%), and the multiple abortion was 39%, while the single abortion 42%.

Keywords

T. gondii, PCR, Prevalence, aborted women, Thi-Qar