1Biology Department, College of Science, University of Misan, Maysan, Iraq
2Biology Department, College of Science, University of Kufa, Najaf, Iraq
3The Islamic University, Babylon, Iraq
4College of Pharmacy, University of Babylon>, Babylon, Iraq
*Corresponding Author: Shaima R. Banoon, Biology Department, College of Science, University of Misan, Maysan, Iraq, Email: shimarb@uomisan.edu.iq
Online published on 13 November, 2019.
Genetic fingerprinting of 19 different isolates of Staphylococcus aureus from wounds, skin, nails and urinary tract infection taken from patients who admitted at AL-Hilla General Teaching Hospital and Babylon Hospital for Maternity and Pediatric, using random amplified polymorphic DNA (RAPD) was carried out. Two (OPY07 and OPX-20) primers showed polymorphism among the isolates tested generating 12 bands, 8 of which were polymorphic with sizes ranging between 250 and 1 kb. All the isolates were classified completely into one major group with nineteen different subgroups. The nineteen different subgroups suggest adaptation of S. aureus in the different host cells. This indicates possible relationship between host origin and genetic variation among S. aureus isolates. The DNA fingerprint defined for each strain of S. aureus could be useful in epidemiological studies, medical diagnosis and the identification of new strains and their origins.
Staphylococcus aureus, RAPD, PCR, Fingerprinting Technique, OPY-07 and OPX-20
