1Department of Veterinary Education, Faculty of Veterinary, Universitas Airlangga, Jl. Mulyorejo, Surabaya, East Java
*Corresponding Author: Kusnoto,Department of Veterinary Education, Faculty of Veterinary, Universitas Airlangga, Jl. Mulyorejo, Surabaya, East Java, 60113, Email: kusnotoo19@gmail.com
Online published on 13 November, 2019.
Agricultural products exposed to endosulfan insecticides potentially lead to organ dysfunction in living organisms that can cause morphological changes in the reproductive system such as a decrease in the number of sperm cells, an inhibition on the testosterone production by the Leydig cells, and a decline in the ascorbic acid levels in the testicles.
This study aimed to determine the effect of vitamin C on testicle weight and the number of Leydig cells in adult male Wistar rats exposed to endosulfan.
This study used 20 male Wistar rats with an average weight of 25 grams as samples, where the rats were equally divided into four groups, i.e., P-, P +, P1, and P2. The testicles of all Wistar rats were harvested on day 19 and then prepared for a microscopic examination with routine H & E staining. The results analyzed by using ANOVA and the Waller-Duncan T-test.
The testicle weight of the Wistar rats administered with endosulfan suggested a significant difference (p <0.05) and had the lowest value compared to the other treatments. The Wistar rats administered with endosulfan and then followed by vitamin C administration did not indicate a significant difference (p <0.05). Vitamin C administration to Wistar rats caused an increase in testicle weight. Conversely, administering endosulfan to the Wistar rats caused a decrease in testicle weight.
Administering vitamin C to Wistar rats exposed to endosulfan could potentially lead to an increase in the testicle weight and the Leydig cells.
Endosulfan, vitamin C, Wistar rats, testicle weight, Leydig cells
