1M.V.Sc. Scholar, Department of Veterinary Pathology, School of Animal Biotechnology
2Sr. Veterinary Pathologist, Department of Veterinary Pathology, School of Animal Biotechnology
3Professor, School of Animal Biotechnology
4Assistant Scientist, School of Animal Biotechnology
5Associate Professor, Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Online published on 24 June, 2014.
Rabies is an enzootic, fatal disease and early detection of this disease is necessary. Till now a reliable diagnosis is possible only after death of animal.
So the present study was undertaken to use the molecular approaches like nested RT-PCR and SYBR Green real time PCR for early diagnosis of rabies in suspected animals using less invasive non neural tissues like skin biopsy and hair follicles.
12 cases of rabies suspected live animals were presented to the Veterinary Clinics, GADVASU, Ludhiana, India.
The sensitivity of nested RT-PCR for hair follicles and skin samples was 50% and 57.1% respectively. A sensitivity of 62.5% and 71.4% was observed with real time PCR in hair follicles and skin samples.
We suggest that non-invasive molecular approaches may be used for the early and accurate diagnosis of rabies in animals.
Diagnosis, Hair Follicle, Skin, Rabies
