1Demonstrator, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Delhi Haridwar By Pass, Meerut, Uttar Pradesh, India
2Professor & Head, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Delhi Haridwar By Pass, Meerut, Uttar Pradesh, India
3Assistant Professor, Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Delhi Haridwar By Pass, Meerut, Uttar Pradesh, India
4Professor & Head, Department of Respiratory Medicine, Subharti Medical College, Swami Vivekanand Subharti University, Delhi Haridwar By Pass, Meerut, Uttar Pradesh, India
*Corresponding author: Mahip Saluja, Department of Respiratory Medicine, Subharti Medical College, Swami Vivekanand Subharti University, Delhi- Haridwar By Pass, Meerut, U.P., 250 005, India. E-mail: drmahip@hotmail.com
Online published on 6 November, 2014.
Conventional methods for detection of Mycobacterium tuberculosis from culture can take up to 8 weeks. Simple and sensitive methods that enhance the detection of Mycobacterium tuberculosis (M.tuberculosis) from clinical specimens are needed, to achieve early diagnosis and effective treatment of pulmonary tuberculosis. To facilitate the rapid detection of Mycobacterium tuberculosis complex and its drug sensitivity pattern by line probe assay was performed in our study.
To assess the prevalence of MDR-TB through a rapid, feasible, reliable method in a tertiary care centre of western Uttar Pradesh.
110 consecutive smear-positive sputum specimens collected from clinically suspicious cases of pulmonary tuberculosis at C.S.S.H, Subharti Medical College, Meerut, India were subjected to Line probe assay which was performed according to the manufacturer's instructions.
Out of 110 AFB positive sputum samples, 30 were both Rifampicin and Isoniazid sensitive, 31 were resistant to both Rifampicin and Isoniazid, 4 were resistant to Rifampicin & sensitive to Isoniazid, 5 were sensitive to Rifampicin & resistant to Isoniazid and 40 were not showing TB complex band in the test strip.
The Line probe assay is a simple, rapid and a reliable molecular method for diagnosing MDR.
Tuberculosis, MDR TB, Molecular diagnosis, INH, RMP
