1Assistant Professor, Department of General Medicine, Santosh Medical College & Hospitals, Ghaziabad, Uttar Pradesh
2Post Graduate IIIrd Year Student, Department of General Medicine, Santosh Medical College & Hospitals, Ghaziabad, Uttar Pradesh
3Professor and HOD, Department of General Medicine, Santosh Medical College & Hospitals, Ghaziabad, Uttar Pradesh
4Assistant Professor, Department of Microbiology, Santosh Medical College & Hospitals, Ghaziabad, Uttar Pradesh
Online published on 26 April, 2016.
Flowcytometric studies facilitate identification of leukemic cells, proper classification of acute leukemias and helps in institution of suitable treatment plans in various types of acute leukemias.
Correct diagnosis and classification of Acute Leukemias on the basis of specific C.D markers.
This study was conducted in the Department of medicine, Santosh medical college and hospital, GZB from February 2012 to January 2014. Patients suspected as acute leukemias on basis of blasts in peripheral smear were included. The blood samples were run in a 4 colors flow cytometry machine for standardized acute leukemia panel as per the guidelines of international flow cytometric association.
A total of 53 patients, suspected of acute leukemias were followed up with flow cytometry. Distribution of HLA DR, was seen in 10 of 15 cases of AML, CD34 was present in 46.7% (7/15) cases of AML and CD117 in 60% (9/15) cases of AML. Expression of myeloid-monocytic markers, MPO, megakaryocyte & NK cell associated antigen-CD61, lymphoid associated antigen & TDT was studied. Various types & subtypes of acute leukemias are classified.
Flowcytometry is a powerful tool for the investigation of normal and neoplastic cells and their classification. It has a great prognostic and therapeutic implication and hence has a bright future and will be used more and more in near future.
FCM- Flow cytometry, AML- Acute Myeloid Leukemia, ALL- Acute leulemoid leukemia
