1Assistant Professor, Department of Pathology, S. N. Medical College, Bagalkot, Karnataka
*Corresponding author: Dr. Deepa S Masur Assistant Professor, Department of Pathology, S. N. Medical College Bagalkot-587101 Karnataka, INDIA e-mail address: drdns11s@gmail.com
Online published on 14 October, 2016.
1) To obtain an ideal Cellblock preparation where in maximal numbers of cells are displayed within a small area.
2)To assess the efficacy of both types of Cellblock techniques in the cytodiagnosis of serous effusions.
3)To identify simple and cost effective Cellblock preparation between the two Cellblock techniques.
The residual fluid sample was divided into two parts and subjected for centrifugation. Part A sediment was subjected for 1 hr and 24 hr fixation in 5 ml of ethyl alcohol and 10% formalin in 1: 1 proportion. To Part B sediment 2 drops of plasma and 2 drops of thrombin was added, mixed well and allowed to clot for 30 sec. The pellet and clot was then processed for paraffin embedding.
Marked cellularity was observed in 80 (72.7%) samples by Plasma-Thrombin method and 28 (25.6%) samples by Formalin method. The Plasma-Thrombin Cellblocks had evenly distributed cells in a small area of the slide. The Formalin Cellblocks had artifactually crowded cells and the material scattered throughout the slide. 14 (12.4%) samples of the Formalin Cellblocks were inconclusive and they did not contain any evaluable cellular material.
Plasma-Thrombin method was effective in producing cellular sections with overall best preservation of architecture. It is simple, cost effective and provide unlimited source of material for further special stains and immunohistochemistry.
Serous effusions, Conventional smear, Cellblock, Plasma-thrombin Cellblock method, Formalin Cellblock method
