1Bhadrak Autonomous College, Bhadrak, Odisha, India
2Ex-Director, RMRC (ICMR) BBSR, Odisha, India
3Professor & HOD Pediatrics, KIMS, KIIT University, Bhubaneswar, Odisha, India
Online published on 14 October, 2016.
Withdrawal of Chloroquine (CQ) had shown a rapid reduction in the frequency of resistance in many countries. The present study was conducted using rapid detection technique to assess the reduction of CQR to determine its current status. The continuous monitoring of CQR will help to establish the point of reintroduction of the drug.
A total of 104 blood samples were collected on Wattman filterpaper-III from the suspected malaria cases in Keonjhar district of Odisha. The PfCRT gene mutation was determined by nested Polymerase Chain Reaction (PCR) method.
The result showed, Plasmodium falciparum Pf) positivity of 48 (46.1%) by PCR assay. The PCR positives were processed for detection of PfCRT (K76T) mutation by nested PCR technique followed by digestion. The result revealed 58.3% of resistance of strain.
The method used in the study will greatly help for screening of population in community based studies. Emergence of Chloroquine susceptibility in the country may give a scope for reusing chloroquine in treatment of Pf malaria.
Plasmodium falciparum (Pf) Malaria, Chloroquine Resistance (CQR), PfCRT K76T
