The Effect of Se'i (Smoked Beef) Toward the Improvement of the Bcl-2 Protein expression on Colon Cells of Balb/c strain Mice as a Carcinogenesis Indicator

Se'i is smoked beef with added nitrite which can precipitate the Bcl-2 protein expression. The purpose of this study was to analyze the effect of se'i toward the improvement of the Bcl-2 protein expression on colon cells of Balb/c strain mice as an indicator of carcinogenesis. This research is true experimental with completely randomized design. Samples were male Balb/c mice, weighing 23.8 gram which was 36 mices, divided into 4 treatment groups P1: Mice were given se'i without nitrite with weight 8.840 mg for 28 days, P2: Mice were given se'i containing nitrite from the modern industry weighing 8.840 mg for 28 days, P3: Mice were given nitrite se'i from home industry weighing 8.840 mg for 28 days and K: Mice fed only standardize feed, and drinking water for 28 days. The examination was done by using immunohistochemical techniques to see the expression of a Bcl-2 protein. The result showed that there was a significant difference of Bcl-2 protein expression on colon cells of male Balb/c strain mice which are given se'i. The LSD test showed that there was a difference of Bcl-2 protein expression between P3, P1 and K. The new finding of this study is the provision of se'i causing the differences in Bcl-2 expression in all treatment groups.