Multiplex PCR targeting mitochondrial DNA to identify local and imported meat species in Al-Diwaniyah City, Iraq

The present study was designed to identify animal source of meat which used for human consumption in Al-Diwaniyah city, Iraq. For this reason, 74 local and imported-meat samples were collected from different stores and retailers in the city throughout one-year period, 2015–2016. a one-reaction multiplex PCR (mPCR) technique was recruited to distinguish five meat sources, beef, lamb, goat, donkey, and pork. The technique principle was based on the amplification of mitochondrial DNA (mtDNA). The results have shown accurate identification of animal source of the meat in which mixing of ground beef and lamb meat was detected in samples collected from local shops and retailers. All imported ground and whole-meat samples were positive for beef. In conclusion, mPCR method could be useful for easy and rapid detection of animal source of meat sold for human consumption to control commercial fraud.