Evaluation of antibacterial activity and qualitative phytochemical analysis of Enteromorpha ralfsii

Filamentous specie of algae (Enteromorpha ralfsii) was isolated from Baher AL-Najaf region Iraq were tested against some bacterial species. methanolic (Hot and Cold) Extracts of Enteromorpha ralfsii were screened to show the antibacterial activity. Four different concentrations (w/vol.) 12.5, 25, 50 and 100 mg/ml were made in each of the above Extracts were loaded on agar plates, containing test bacteria, staphylococcus aureus, staphylococcus epidermidis, Bacillus substilis, Escherichia coli, Klebseilla sp. and pseudomonas aeruginosa. The hot methanolic extract was efficiency for making extract that showed good zone of inhibition in bacterial species maximum up to 23 mm than the lower value was 9 mm. Chemical analyses showed that the active chemical compounds for hot methanolic extract alga (Enteromorpha ralfsii) extract was contains Tannins, Terpenoid, Flavonoids and Saponins. While Alkaloids, Glycosides and Phenols, were absent. The methanolic extract was further chemically characterized by using GC-MS in order to be tentative identify the compounds responsible for such activities, The main compositions were found in hot methanolic crud extract, these were: Nonadecane (16.2%) is an alkane hydrocarbon, Alkanes while Pentadecane represented (39.6%) this compounds which had antimicrobial activity. These results indicate that the methanolic extract of Enteromorpha ralfsii exhibited appreciable antimicrobial activity and could be a source of valuable bioactive materials for health products.