1Department of Anatomy, IMS and SUM Hospital, Siksha ‘O’ Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
2Department of Anatomy, SCB Medical College, Cuttack, Odisha, India
3Directorate of Medical Researach, Department of Pharmacology, IMS and SUM Hospital, Siksha ‘O’ Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
4Department of Pharmacology, IMS and SUM Hospital, Siksha ‘O’ Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
Online published on 7 December, 2018.
In vitro study of the toxic activity of aqueous extract of heart wood of Pterocarpus marsupium on lymphocytes of human cord blood. Pterocarpus marsupium is a traditional drug used as an anti-diabetic agent for ages in India. Many research works have proved its efficacy as antidiabetic medication, but there is hardly any report regarding its toxicity on from the available literature. This study was undertaken to conduct its toxicity study.
Lymphocytes from human cord blood were cultured in Dulbecco's modified eagle's medium. The toxicity of the aqueous extract of heart wood Pterocarpus marsupium was assessed by Acridine orange/Ethidium Bromide(AO/EB) Staining method as well as by 3-[4, 5-dimethylthiazol-2-yl]2, 5-diphenyl tetrazolium bromide(MTT) assay. The results were analyzed statistically.
Pterocarpusmarsupium extracts showed that extract amounts up to 50 mg/ml are found safe based on the absence of abnormal blood cell counts and blood chemistry values and the absence of extract-related adverse events
Heart wood of Pterocarpus marsupium, lymphocyte, toxicity, cord blood
