1Ph. D Scholars at Bharath Institute of Higher Education and Research, Chennai
2Professor, Department of Microbiology, Srilaxmi Narayana Institute of Medical Sciences, Pondicherry
*Corresponding author: P Suresh, Ph. D Scholars at Bharath Institute of Higher Education and Research, Chennai, email: sureshraja25@gmail.com
Online published on 7 December, 2018.
Lactobacilli are involved in the microbial homeostasis in the gastrointestinal tract and female genital tract. Due to the high prevalence of fungal and bacterial infections of the female genital tract and the emerging resistance of microbial pathogens to various antimicrobial agents, alternative measures to control these infections are increasingly felt by the scientific community. Lactobacillus was considered as probiotic used in controlling some bacterial infections because of the property of Lactobacillus exhibiting antimicrobial activity and thus augmenting the therapy by antimicrobial drugs.
Many studies were undertaken to evaluate the probiotic properties of Lactobacillus against germ tube positive Candida spp. namely C.albicans & C. dubliniensis. The probiotic potential was investigated by using the following criteria: (i) adhesion to host epithelial cells and mucus, (ii) biofilm formation, (iii) co-aggregation with bacterial pathogens, (iv)inhibition of pathogen adhesion to mucus and HeLa cells, and (v) antimicrobial activity. Documented studies reveal lactobacilli adhered to mucin, co-aggregated with all genital microorganisms, and displayed antimicrobial activity. L. fermentum produced a moderate biofilm and a higher level of co-aggregation and mucin binding. The displacement assay demonstrated that all Lactobacillus strains inhibit C.albicans & C.dubliniensis binding to mucin (p < 0.001), likely due to the production of substances with antimicrobial activity.
In this study Clinical isolates of C.albicans & C.dubliniensis associated with vaginal candidiasis were inhibited by L. fermentum. Our data suggest that L. fermentum isolated from two days fermented goat milk is a potential probiotic candidate, particularly to complement candidiasis treatment.
Lactobacillus fermentum isolated from two days fermented Goat milk had good effect preventing the growth of Germ tube positive Candida species (Candida albicans and Candida dubliniensis).
Candida albicans, Candida dubliniensis, Vitek-II compact system, and YST, YS02 (BIOMERIX IN INDIA)
