The effects of extract andaliman fruit (Zanthoxylum acanthopodium Dc) to CAMP mRNA expression and bacterial load in mice balb-C after Gardnerella vaginal Infection

This study aims to analyze the effects of extract Andaliman Fruit /Zanthoxylum Acanthopodium Dc (EZa) to CAMP mRNA expression and bacterial load in Mice Balb-C before and after Gardnerella vaginalis infection. Experiment to Balb-c mice consists of three groups; Control groups, given EZa 5 days groups and EZa 7 days groups prior Gardnerella vaginalis inoculation. Bacterial number and colonies, mRNA CAMP expression, were examined 3 and 5 days after inoculation of Gardnerella vaginalis. Data showed that administration of EZa extract for 7 days before Gardnerella vaginalis inoculation had a significant effect both on the decrease in bacterial number and bacterial colonies as well as in enhancing CAMP mRNA expression. The five-day EZa extract reduced the number of bacteria and increased mRNA expression significantly but was less effective in reducing the number of bacterial colonies. Controls group that was not given anything had significant increases in bacterial numbers and colonies, and increased CAMP expression was higher compared to another group. Conclusion: this study found Extract of Zanthoxylum acanthopodium DC (EZa) indicated as a potential anti-bacterial and immunomodulator especially to Gardnerella Vaginalis infection.