Gene expression of pulp cells from supernumerary tooth through the passages

Evaluate the gene expression of the human dental pulp cells harvested from supernumerary tooth through the passages by using quantitative reverse transcription PCR.

Stem cells were harvested from the extracted supernumerary tooth. Primary cells were incubated at 37° for 7 days. When the cells reached 80% confluency, they were subcultured for the next passage. The same subculture was performed until the 16th passage. Total RNA of dentin sialophosphoprotein(DSPP), ALP, dentin matrix acidic phosphoprotein 1(DMP-1), osteocalcin, and osteonectinwere extracted and quantitative reverse transcription PCR was used to measure gene expression.

The supernumerary tooth is surgically removed at a relatively early age. The tooth can be obtained in an aseptic condition, and it could be extracted at a scheduled time so it could be a new donor of tooth stem cell harvesting. We performed this study using dental stem cells collected from supernumerary teeth. From the 1st passage to 16th passage, more DMP-1 was observed in early passage compared to late passage. More ALP, Osteocalcin, Osteonectin were observed in late passage compared to early passages. DSPP showed constant expression during all passages. Gene expression of ALP, Osteonectin, Osteocalcin, DMP-1, and DSPP showed no difference in the undifferentiated and differentiated groups and between passages through 1^st to 16^th also.

Stem cells from supernumerary tooth showed high gene expression potential although it reached more than the 8th passage.