1Research Scholar, Dept. of Preventive and Social Dentistry, School of Dentistry, Kyung Hee University, Republic of Korea
2Associate Professor, Dept. of Dental Hygiene, College of Health Science, Dankook University, Republic of Korea
*Corresponding Author: Jong-Hwa Jang, Associate Professor, Dept of Dental Hygiene, College of Health Science, Dankook University, Korea, Email: jhj@dankook.ac.kr
Online published on 7 December, 2018.
This study aimed to assess the effect of oral spray containing pine needle extract in suppressing halitosis, by analyzing the concentration of volatile sulfur compounds(VSCs) inducing halitosis and organic acids within saliva. The concentration of VSCs inside the oral cavity after the spray usage was measured using Sensor Gas Chromatograph ODSA-P2, and the analysis of organic acids within saliva was performed using HPLC Dionex ion chromatography system (GP50 gradient pump, ED 50 conductivity detector) with mobile phase under following conditions: NaOH 100mM with flow rate of 1.0mL/min, injection volume of 10㎕, and temperature of 20℃. In the experimental group, the levels of H2S and CH3SH were remarkably reduced by 80.87% and 89.42% respectively. In addition, the analysis of organic acids within saliva showed suppressive effect by 63.46–97.05% after 4 weeks. Therefore, it seems that using oral spray mist containing pine needle extract can partially suppress VSCs and organic acids within saliva that are associated with halitosis.
HPLC, halitosis, oral spray, pine needle extract, saliva
