1Research Scholar, Medical Research Laboratory, IMS and SUM Hospital, Siksha O Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
2Professor Department of Community Medicine, IMS and SUM Hospital, Siksha O Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
3Professor Department of Medicine, IMS and SUM Hospital, Siksha O Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
4Assistant Professor, Medical Research Laboratory, IMS and SUM Hospital, Siksha O Anusandhan Deemed to be University, K8, Kalinga Nagar, Bhubaneswar, Odisha, India
*Corresponding Author Dr. Mahesh Chandra Sahu, PhD, NPDF, Assistant Professor, Medical Research Laboratory, IMS and SUM hospital, Bhubaneswar, E-Mail: mchsahu@gmail.com
Online published on 13 December, 2018.
Urinary Tract Infection (UTI) prevails to be the most common infection among all nosocomial infections. Due to indiscriminate, misuse and over use of antibiotics in ICU, it reveals multidrug resistant (MDR) strains. Proper antibiograms study should be carried out before empirical therapy.
In this study, we aimed to investigate the common isolates of UTI and their drug susceptibility test and identified the gene responsible for resistance to tigecycline antibiotic.
This study was executed with routine microscopic, identification of organisms and drug sensitivity of isolates from 270 urine samples for a period of six months at Institute of Medical Science and SUM Hospital, Bhubaneswar, Odisha. Bacteria were identified with universal primer (16S rRNA). Bacteria having tigecycline resistant genes were identified using specific primers.
From 270 urine samples, 128 showed significant growth. P. aeruginosa was common isolate, followed by S. aureus, E. coli. In case of fungus, C. albicans was common. Bacteria were identified using 16S rRNA primer (8F, 1541R). Drug susceptibility pattern reveled highly resistant to imipenem and azithromycin in case of Gram negative bacteria. However, colistin was sensitive to all. In case of Gram positive cocci, ciprofloxacin and penicillin remained high resistance but chloramphenicol was the most sensitive. For fungal isolates amphotericin and fluconazole remained highly resistant while itraconazole showed high sensitive. By using specific primers (SoxS, acrA and tolC), tigecycline resistant genes were identified in E. coli and Shigella species.
Empirical use of antibiotic should not be practiced while prescribing drugs. Proper identification and drug susceptibility pattern of organisms should be conducted before prescribing a drug. PCR method should be implemented for early detection of antibiotic resistance patterns.
Nosocomial infection, MDR, 16S rRNA, Tigecycline, Empirical therapy, ICU
