Improving an Illumination System in the Microscopic Imaging of Nuclear Tracks Using Light Emitting Diode

In this study, images vision of nuclear tracks was increased using two methods. The frst is to design a new light system based on Light-Emitting Diodes (LED) light instead of traditional fuorescent light used in the optical microscope. Due to the high dynamic range of the LED light, a high vision and an extra number of tracks were obtained during shooting. The second method is to establish a new scale depended on counting nuclear tracks. That is called No-Reference Image Quality Assessment Based on Wavelet Transform (NIQWT) scale. The quality of captured images was evaluated and no-reference scales like Entropy of First Derivative (EFD), Average Gradient (AG), the Measure of Enhancement by Entropy (EMEE), and (NIQWT) were calculated to be recommended. Then a good correlation coeffcient was obtained for these scales. The best correlated coeffcient 0.9032 was for (NIAWT) scale. Whereas, the statically results illustrated photography when using LED light much better than Florescent light in the optical microscopy. Therefore, these processes led to augment numbering of nuclear tracks discovered.