1Dept. of Physiology, College of Korean Medicine, Semyung University, Jecheon, Chungcheongbuk-do, KS001, Republic of Korea
2Dept. of Pediatrics, Hospital of Korean Medicine, Pusan National University Yangsan, KS012, Republic of Korea
3Dept. of Anatomy, College of Korean Medicine, Semyung University, Jecheon, Chungcheongbuk do, KS001, Republic of Korea
*Corresponding Author: Ho-Hyun Kim, Professor, Dept. of Physiology, College of Korean Medicine, Semyung University, Jecheon, Republic of Korea. Email: binbae@semyung.ac.kr
Online published on 2 February, 2019.
In recent times, the number of men with osteoporosis has increased, and interest on this topic has also increased. This research was conducted to look into the regulatory effects of Gami-Shinkiwhan on osteoporosis induction.
The experimental subjectsweresplited into three groups: a control group consisting of 8-week-old male ICR mice that had undergone no treatment, an osteoporosis-elicited group(OPE group) consisting of 24-month-old ICR male mice that had undergone no treatment, and a Gami-Shinkiwhan treatment group(GS group) consisting of 24-month-old ICR male mice that had undergone Gami-Shinkiwhan(0.56 g/kg/day)treatment for 10 weeks. After the experiment, the mice from all the experimental groups were dissected, and they were analyzed through histochemical and immunohistochemical methods.
Compared to the OPE group, in the GS group, the osteoporosis-induced area was decreased, the RANKL positivity was decreased, and the osteoprotegerin (OPG)positivity was increased. Compared to the OPE group, in the GS group, the bone matrix distribution was increased and the osteocalcin (OPC) and osteopontin (OPN) positivity was increased.
The outcomes of this research suggest that Gami-Shinkiwhan inhibits osteoclastogenesis, by decreasing RANKL levels and increasing OPG levels, and increases bone matrix distribution and OPC and OPN activities in the osteoblast.
Gami-shinkiwhan(GS), Osteoporosis, Osteoprotegerin(OPG), Reaction of activation of nuclear factor kappa B ligand (RANKL), Osteocalcin(OPC), Osteopontin(OPN)
