Indian Journal of Public Health Research & Development
  • Year: 2018
  • Volume: 9
  • Issue: 3

Analysis of Methanolic Extract of Fusarium Chlamydosporum using GC-MS Technique and Evaluation of its Antimicrobial Activity

1Department of Biology, College of Science, University of Al-Qadisiyah, Hillah city, Iraq

2Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq

3Department of Biology, College of Science for women, University of Babylon, Hillah city, Iraq

*Corresponding Author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq; Phone: 009647716150716; E-mail: imad_dna@yahoo.com

Online published on 3 April, 2018.

Abstract

Chromatography is the term used to describe a separation technique in which a mobile phase carrying a mixture is caused to move in contact with a selectively absorbent stationary phase. The objectives of this study were analysis of the secondary metabolite products and evaluation antimicrobial activity. Bioactives are chemical compounds often referred to as secondary metabolites. Eleven bioactive compounds were identified in the methanolic extract of Fusarium chlamydosporum. DL-Arabinose, D-Glucose, 6-O-α-D-galactopyranosyl, α-D-Glucopyranoside, O-α-D-glucopyranosyl, 5-Hydroxymethylfurfural, N-(4, 6 Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylidenea, 1H-Purin-2-amine, 6-methoxy-N-methyl, 2-Methyl 9-β-d-ribofuranosylhypoxanthine, 1-Hexadecanesulfonic acid, 3, 5,-dichloro-2, 6-dimeth, Methyl-6, 7 benzoisoquinoline, Undeca-2, 4, 6, 8, 10-pentaenal, 11-(2-furyl)-, oxime, and 2-Bromotetradecanoic acid. Malva sylvestris was very highly active 6.85±0.25 mm. The results of anti-fungal and anti-bacterial activity produced by Fusarium chlamydosporum showed that the volatile compounds were highly effective to suppress the growth of Aspergillus terreus (6.09±0.21) and Staphylococcus aureus (5.99±0.19) mm.

Keywords

Anti-Microbial, Fusarium chlamydosporum, GC-MS, Secondary metabolites