1Department of Biology, College of Science for women, University of Babylon, Hillah city, Iraq
2Department of Biology, College of Science, University of Al-Qadisiyah, Hillah city, Iraq
3Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq
*Corresponding Author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq Phone: 009647716150716 E-mail: imad_dna@yahoo.com
Online published on 3 April, 2018.
Raphanus raphanistrum is an edible root vegetable of the Brassicaceae family. The objective of this study were analysis of the secondary metabolite products. Twenty bioactive compounds were identified in the methanolic extract of Raphanus sativus. GC-MS analysis of Raphanus sativus revealed the existence of the Raphanus sativus leaves proved the presence of alkenes, alkanes, and alkyl halides which shows major peaks at 844.82, 937.40, 1012.63, 1022.27, 1066.64, 1143.79, 1217.08, 1234.44, 1361.74, 2330.01, 2341.58, 2358.94, and 2854.65. In the current study, the anti-microbial activity of Raphanus sativus methanolic extract was evaluated by determining the zone of inhibition against nine bacteria and eight fungi and yeast. Maximum zone formation was against Staphylococcus aureus (6.75±0.25). Raphanus sativus was very highly active against Aspergillus terreus (6.99±0.26).
FT-IR, GC-MS analysis, Raphanus sativus, Anti-Bacterial, Anti-Fungal Activity
