Determination of Alkaloid Compounds of Datura Stramonium Using Gc-Ms and Ftir and Evaluation of its Antibacterial, Antifungal and Anti-Diabetic Activity

The aim of this study is to assess the compounds of alkaloids extracts from the leaves of Datura stramonium, which can be the basis for the synthesis of new antibiotics. In this study, the alkaloid compounds of Datura stramonium have been evaluated. GC-MS analysis of alkaloid leaves ethanolic extract of Datura stramonium revealed the existence of the Ethyl iso-allocholate, D-asycarpidan-1-methanol, acetate (ester), 3-(1, 5-Dimethyl-hexyl)3a, 10, 10, 12b-tetramethyl1, 2, 3, 3a 4, 6, 8, 9, 10, 10a, 11, 12, 12a, 12b-tetradec-ahydro-benzo[4, 5] cyclohept, 2, 7-Diphenyl-1, 6-dioxopyridazino [4, 5: 2, 3] pyrrolo[4, 5-d] pyridazine, 3, 8, 8-Trimethoxy-3-piperidyl-2, 2-benaphthalene-1, 1, 4, 4-tetrone, [5β] Pregnane3, 20β-diol, 14α, 18α-[4methyl, 3-oxo-[1-oxa-4-azabutane-1, 4-diyl], diacetate, 1-Monolinoleoylglycerol trimethylsilyl ether, and 17-[1, 5-Dimethylhexyl]-10, 13-dimethyl-3sstyrylhexadecahydrocyclopent a[a]phenathren-2-one. The FTIR analysis of Datura stramonium leaves proved the presence of alkanes, and alkyl halide, Amine, Aldehyde, and Alkane which shows major peaks at 875.68, 1018.41, 1149.57, 1238.3, 1269.16, 1740.51, 2330.01, 2341.58, 2854.65, 2910.58, 2926.01, and 3010.88. Clinical pathogens were selected for antibacterial activity namely, Bacillus subtilis, Salmonella typhi, Staphylococcus aureus, Proteus mirabilis, Escherichia coli, Streptococcus faecalis, Streptococcus pyogenes and Klebsiella pneumonia. Datura stramonium has maximum zone against Staphylococcus aureus (5.895±0.20).