Bioactive Chemical Analysis of Enterobacter aerogenes and Test of its Anti-fungal and Anti-bacterial Activity and Determination

Enterobacter is a genus of Gram-negative, rod shaped, facultative anaerobic, and non-spore forming microbes of family Enterobacteriaceae. Enterobacter aerogenes (E. aerogenes) is well known opportunistic bacteria emerged as nosocomial pathogen in intensive care unit patients. The objectives of our research were analysis of the secondary metabolite products and evaluation antimicrobial activity. Twenty seven bioactive compounds were identified in the methanolic extract of Enterobacter aerogenes. GC-MS analysis of Enterobacter aerogenes revealed the existence of the Butanoic acid, 3-methyl, 2, 6-Lutidine-4-[benzyloxy]-3, 5-dichloro, 1-Propaneamine, 3-(methylthio), Butoxyacetic acid, 3-Hydroxy-2-methylthio-3-phenylpropanoic acid, 1-Deoxy-d-arabitol, Benzeneethanamine, 2-Butanamine, (S), Thiazole, 2-amino-5-methyl, Thiophene, 2, 5-bis(1, 1-dimethylethoxy), Propanedinitrile, 2-bis(3, 3-dimethyl-2-oxobutylthio), Carbamic acid, hydroxyl-, ethyl ester, 3-Aminopiperidin-2-one, dl-Cystathionine, Ethanol, 2-(diethylamino)-, N-oxide, 2, 4-Heptadien-6-yn-1-ol, (E, E), Xanthine, 1, 3-dipropyl-8-[4-[β-[(benzyloxycarbonyla, Isoquinoline, 1-ethyl and Cyclohexanecarboxylic acid, 2-phenylethyl ester. Coriandrum sativum was very highly active (6.75±0.22) mm. The results of anti-fungal activity produced by Enterobacter aerogenes showed that the volatile compounds were highly effective to suppress the growth of Candida albicans (5.717±0.18).