1Ministry of Public Health, Maysan Health Department, Mesan Governorate, Iraq
2Department of Biology, College of Science for Women, University of Babylon, Hillah city, Iraq
3Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq
*Corresponding author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq; Phone number: 009647716150716; E-mail: imad_dna@yahoo.com
Online published on 29 May, 2018.
Pseudomonas fluorescens is a common gram-negative, rod-shaped bacterium [1]. It belongs to the Pseudomonas genus. The objectives of this study were analysis of the secondary metabolite products and evaluation antimicrobial activity. Twenty four bioactive compounds were identified in the methanolic extract of Pseudomonas fluorescens. GC-MS analysis of Pseudomonas fluorescens revealed the existence of the cis-5, 8, 11, 14, 17-Eicosapentaenoic acid, 12, 15-Octadecadiynoic acid, methyl ester, 7-epi-cis-sesquisabinene hydrate, α-D-Glucopyranoside, O-α-D-glucopyranosyl, Acetamide, N-methyl-N-[4-[2-acetoxymethyl-1-pyrrol, Acetamide, N-methyl-N-[4-[2-fluoromethyl-1-pyrrolid, Phen-1, 4-diol, 2, 3-dimethyl-5-trifluoromethyl, Geranyl isovalerate, Quinazoline, 4-methyl, Pentetic acid, trans-13-Octadecenoic acid, 9-Hexadecenoic acid, 13-Hexyloxacyclotridecan-2-one, 7-Methyl-Z-tetradecen-1-ol acetate, cis-13-Eicosenoic acid, Didemin B, Hexadecanoic acid, 1-(hydroxymethyl)-1, 2-ethaned, and Ethyl iso-allocholate. Cassia angustifolia (Crude) was very highly active (6.007±0.20) mm against Pseudomonas fluorescens. The results of anti-fungal activity produced by Pseudomonas fluorescens showed that the volatile compounds were highly effective to suppress the growth of Trichoderma horzianum (5.019±0.18). Pseudomonas fluorescens produce many important secondary metabolites with high biological activities.
Anti-Microbial, Pseudomonas fluorescens, GC-MS, Secondary metabolites
