1Ministry of Public Health, Maysan Health Department, Mesan Governorate, Iraq
2Department of Biology, College of Science for Women, University of Babylon, Hillah City, Iraq
3Biomedical Science Department, University of Babylon, College of Nursing, Hillah City, Iraq
*Corresponding author: Imad Hadi Hameed Biomedical Science Department, University of Babylon, College of Nursing, Hillah city, Iraq; Phone number: 009647716150716; E-mail: imad_dna@yahoo.com
Online published on 29 May, 2018.
The objectives of this study were analysis of the secondary metabolite products and evaluation antibacterial and antifungal activity. Bioactives are chemical compounds often referred to as secondary metabolites. Twenty nine bioactive compounds were identified in the methanolic extract of Citrus aurantifolia. The identification of bioactive chemical compounds is based on the peak area, retention time molecular weight and molecular formula. GC-MS analysis of Citrus aurantifolia revealed the existence of the Thieno[2, 3-c]furan-3-carbonitrile, 2-amino-4, 6-dihydro-4, 4, 6, 6, Furfural, 2-Vinyl-9-[3-deoxy-β-d-ribofuranosyl]hypoxanthine, 2-Myristynoyl pantetheine, 2, 5-Furandione, dihydro-3-methylene-, Cyclohexene, 1-methyl-4-(1-methylethenyl)-, (S)-, O-Acetyl-4-hydroxyproline, 1, 5, 5-Trimethyl-6-methylene-cyclohexene, Acetic acid, 2-(1-buten-3-yl)-2-nitro-, ethyl ester, Methyl 3-hydroxytetradecanoate, L-α-Terpineol, 4-Methyl itaconate Glycyl-D-asparagine, 2(3H)-Benzofuranone, hexahydro-7a-methyl-, 7-Oxa-2-oxa-7-thiatricyclo[4.4.0.0(3, 8)]decan-4-ol, Cholestan-3-ol, 2-methylene-, (3β, 5α)-, Formic acid, 3, 7, 11,-trimethyl-1, 6, 10-dodecatrien-3-yl ester, 7-epi-cis-sesquisabinene hydrate, 2, 5-Cyclohexadien-1-one, 3, 5-dihydroxy-4, 4-dimethyl-2-(1-oxo, Pyrrolidin-2-one-3β-(propanoic acid, methyl ester), 5-methylen, D-Fructose, diethyl mercaptal, pentaacetate, n-Hexadecanoic acid, 2H-1-Benzopyran-2-one, 5, 7-dimethoxy-, Dihydroxanthin, Oleic acid, Octadecanoic acid, Phorbol, 9-Octadecenamide, (Z)-and 9-Octadecenamide. Clinical pathogens were selected for antibacterial activity namely, Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Klebsiella pneumonia, and Pseudomonas eurogenosa. Citrus aurantifolia has maximum zone against Escherichia coli 5.66±0.21.
Antimicrobial activity, Bioactive compounds, Fruit, GC-MS, Citrus aurantifolia
