The Effect of Blood Lead Levels on Malondialdehyde as an Indicator of Oxidative Stress in Workers of Gas Station in Sleman

This study aims to determine the effect of lead exposure on MDA levels as an indicator of the risk of oxidative stress associated with damage to cell membranes, DNA, RNA, and brain cells at gas station officers in Sleman.

This research is an observational analytic study with quantitative research and using Cross-Sectional design on 43 workers of gas station that selected by inclusion and exclusion criteria in 3 places located in Sleman.

The average blood lead level on subjects are 62.174 μg/L with standard deviation 23.602. The lowest lead level is 16.707 μg/L and the highest is 118.503 μg/l. Average levels of MDA is 5.869 μg/L with standard deviation 1.232. The lowest levels of MDA is 3.53 μg/L and the highest is 7.97 μg/. Pearson correlation test results showed that there is a highly significant correlation between blood lead levels and Malondialdehyde levels (p = 0.0000) (r = 0.8432). The multivariate analysis showed that the blood lead level was significant predictor (p<0, 05) toward MDA levels with effective contribution of 69.6%. The linier regression equation is “MDA = 3.1605 + 0.000043 * lead levels”. The equation can be used to predict the value of MDA levels taking into account the levels of lead, for example blood lead level of 10.000 μg/L then MDA levels predicted of 3.590 μmole/L. In this pathway, Aminolevulinic Acid Dehydratase (ALAD) enzyme is one of most vulnerable to lead toxicity. Inhibition of δ-ALAD by lead will cause high levels of Aminolevulinic Acid (ALA) in the blood. High levels of ALA in blood will increase production of ROS.

Blood lead levels and malondialdehyde levels in employee of gas station (SPBU) has exceeded the normal limit which average blood lead level are 62, 174μg/L and the levels of MDA are 5.86 μmole/LA.