1Health Analyst Department, Collenge of Mandala Waluya Health Science, Kendari, Southeast Sulawesi, Indonesian
2Biology Department Mathematics and Natural Sciences Faculty, Brawijaya University, Malang, East Java, Indonesia
The aim of this study was to find indigenous bacteria that have the potential to produce protease that can be applied in isolation of collagen from snakehead fish scale.
This study consists of isolation of proteolytic bacteria using skim milk agar (SMA) media, pathogenicity test using blood agar media, measurement of enzyme protease activity quantitatively, extracellular protease production based on the optimum production time and collagen isolation of fish scales using selected bacterial protease. Based on protease activity and pathogenicity test, 3 isolates that have the highest protease activity and non-pathogenic were found namely SB38 (658.3 U/mL), SB16 (618.3 U/mL) and SB23 (613.8 U/mL). Proteins produced by collagen isolation of snakehead fish scales using 3 isolates showed that the powder scales have shorter extraction time (12 hours) than the whole scales. The protein levels extracted from powder scales within 12 hours using isolate SB38, SB23 and SB16 obtained 14.89 μg/mL, 13.24 μg/mL and 12.41 μg/mL respectively. Extraction using isolate SB38 in whole scale and powder scales and extraction using isolate SB16 in powder scales show a clear pattern of protein bands and similar to band pattern of collagen which consists of coincident α-1 and α-2 (90–120 kDa) chains. Bacterial isolate SB16 and SB38 are isolates that produce potential protease in the process of collagen extraction of snakehead fish scales.
Proteolytic bacteria, protease, collagen, snakehead fish scale
