The Anti-Fertility and Cytotoxicity Effects of Cyproterone Acetate and Phenolic Extract of Hibiscusrosa Sinensis linn flowers in Male Albino Rats

The current study was designed to investigate the anti-fertility activity of Cyproterone acetate CPA and phenolic extract of Hibiscus rosa sinensis L. flowers and their cytotoxicity on the splenic lymphocytes (In vivo) and (In vitro) of male albino rats. The animals were divided into two groups, the first group was treated with CPA at dose 5mg/kg/day for 50 days and the second group treated with phenolic extract of H..rosa sinensis flowers in dose 300 mg/kg/day for 60 days. The control group was also divided into two groups, the first group treated with corn oil for 50 days and the second treated with tap water for 60 days. The results showed a significant decrease (p<0.05) in the sperm parameters, and a significant increase (p<0.05) in percent of sperms abnormalities and the decrease in the sexual efficiency of males treated with CPA, whereas the sexual efficiency not affected in the group of rats treated with the phenolic extract. The pregnancy didn^’t occur in females mated with males treated with CPA until two weeks after the end of the treatment period. While the pregnancy didn^’t occur in females mated with phenolic extract of H. rosa sinensis flowers until five weeks after the end of the treatment period, also the results showed a significant increase in proliferation index (PIX) of splenic lymphocytes of albino rats (In vivo).