Cytotoxic and Apoptotic Effects of Phenolic Extract of Hibiscus Rosa Sinensis Linn Flowers Against Cancer Cells And Normal Cells

Phenolic extract of Hibiscus rosa sinensis Linn flowers was evaluated for their cytotoxicity and apoptosis induction in human testicular cancer cell line Tera-1 and macrophage cancer cells from an adult BALB/c strain mouse male RAW 264.7 as compared to normal human hepatic cells WRL-68. The results showed that the phenolic extract of H. rosa sinensis flowers were effective on all cancer cell lines used in this study and Tera-1 cells with IC50 value of 23.496 mg/ml were more sensitive toward phenolic extract of H. rosa sinensis flowers than RAW264.7 cells with IC50 value 31.841 mg/ml, whereas the WRL-68 cells with IC50 value 498.884 were not affected by phenolic extract. The Multi-Parameters Cytotoxicity Activity of extract on Tera-1 cell line showed a significant decrease (P<0.05) in valid cell count, nuclear intensity and mitochondrial membrane potential (MMP) and a significant increase in membrane permeability and cytochrome C releasing, therefor the phenolic extract of H. Rose sinensis have anti-tumor activity, especially on testicular cancer cells.