1Department of Biology, College of Science, University of Babylon, Hillah city
2Biotechnology Research Center, University of Al-Nahrin, Baghdad city
3Department of Biology, College of Science, University of Babylon, Hillah city, Iraq
*Corresponding Author: Al-Saily, H. M. Department of Biology, College of Science, University of Babylon, Hillah city, Iraq, Email: halamohy77@gmail.com
Online published on 21 July, 2018.
Phenolic extract of Hibiscus rosa sinensis Linn flowers was evaluated for their cytotoxicity and apoptosis induction in human testicular cancer cell line Tera-1 and macrophage cancer cells from an adult BALB/c strain mouse male RAW 264.7 as compared to normal human hepatic cells WRL-68. The results showed that the phenolic extract of H. rosa sinensis flowers were effective on all cancer cell lines used in this study and Tera-1 cells with IC50 value of 23.496 mg/ml were more sensitive toward phenolic extract of H. rosa sinensis flowers than RAW264.7 cells with IC50 value 31.841 mg/ml, whereas the WRL-68 cells with IC50 value 498.884 were not affected by phenolic extract. The Multi-Parameters Cytotoxicity Activity of extract on Tera-1 cell line showed a significant decrease (P<0.05) in valid cell count, nuclear intensity and mitochondrial membrane potential (MMP) and a significant increase in membrane permeability and cytochrome C releasing, therefor the phenolic extract of H. Rose sinensis have anti-tumor activity, especially on testicular cancer cells.
Cytotoxic, apoptotic, Hibiscus rosa sinensis cancer cells, normal cells
